1JB2
CRYSTAL STRUCTURE OF NTF2 M84E MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.290, 79.444, 41.747 |
| Unit cell angles | 90.00, 103.80, 90.00 |
Refinement procedure
| Resolution | 22.300 - 2.000 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.502 |
| Number of reflections | 14697 | |
| <I/σ(I)> | 10.5 | 3.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
