1JAZ
Crystal Structure of Monoclinic Form of D90E Mutant of Escherichia coli Asparaginase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.104 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.118, 133.076, 62.565 |
| Unit cell angles | 90.00, 108.78, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.270 |
| R-factor | 0.1828 |
| Rwork | 0.180 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MONOMER A FROM NATIVE L-ASPARAGINASE II STRUCTURE - PDB CODE: 3ECA |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.098 | 0.143 |
| Number of reflections | 26057 | |
| <I/σ(I)> | 28.9 | 6.2 |
| Completeness [%] | 98.6 | 87.3 |
| Redundancy | 4.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG MME 550, MES, zinc sulfate, pH 6.5, 292 K, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
