1JAX
Structure of Coenzyme F420H2:NADP+ Oxidoreductase (FNO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.004 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.640, 72.140, 70.400 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 19.860 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.22100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model from hexagonal crystals (P6(2)22) of FNO solved by MAD phasing but not refined |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.480 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.710 * |
| Number of reflections | 35340 * | |
| <I/σ(I)> | 17.2 | 11 |
| Completeness [%] | 98.5 * | 99.2 * |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 4 * | 30% MPD, 0.1 M Na ac, 0.05 M MgCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 12 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 30 (%) | |
| 3 | 1 | reservoir | acetate/NaOH | 0.1 (M) | pH4.6 |
| 4 | 1 | reservoir | 0.4 (M) |
