1JAD
C-terminal Domain of Turkey PLC-beta
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97949, 0.97940, 0.97526 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.111, 51.928, 79.350 |
| Unit cell angles | 101.07, 96.96, 100.70 |
Refinement procedure
| Resolution | 20.000 * - 2.400 |
| R-factor | 0.254 * |
| Rwork | 0.254 |
| R-free | 0.29300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 14.800 * |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.079 | 0.171 |
| Number of reflections | 20867 | |
| <I/σ(I)> | 12.6 | 6.2 |
| Completeness [%] | 91.8 | 75.1 |
| Redundancy | 5.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | PEG 2000, bis-tris, lithium sulfate, glycerol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 | 12-16 (%(w/v)) | |
| 2 | 1 | reservoir | Bis-Tris | 80 (mM) | pH5.7-6.3 |
| 3 | 1 | reservoir | ammonium sulfate | 60-120 (mM) | |
| 4 | 1 | reservoir | glycerol | 10 (%(v/v)) |
