1J9Y
Crystal structure of mannanase 26A from Pseudomonas cellulosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8374 |
Spacegroup name | P 41 |
Unit cell lengths | 93.240, 93.240, 54.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.500 - 1.850 |
R-factor | 0.182 * |
Rwork | 0.182 |
R-free | 0.20000 |
Structure solution method | MIR |
RMSD bond length | 0.013 |
RMSD bond angle | 0.029 |
Data reduction software | DENZO (/ SCALEPACK) |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.850 |
High resolution limit [Å] | 1.850 | 1.800 |
Rmerge | 0.033 | 0.077 |
Number of reflections | 40381 | |
<I/σ(I)> | 29.9 | 10.2 |
Completeness [%] | 99.8 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG MME 550, zinc sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG550 MME | 26 (%) | |
2 | 1 | reservoir | zinc sulfate | 0.012 (M) | |
3 | 1 | reservoir | MES | 0.1 (M) |