1J9E
Low Temperature (100K) Crystal Structure of Flavodoxin D. vulgaris S35C Mutant at 1.44 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.550, 50.550, 138.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.370 - 1.440 |
| R-factor | 0.136 * |
| Rwork | 0.136 |
| R-free | 0.15600 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.370 | 16.370 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.040 | 0.046 |
| Total number of observations | 29127 * | |
| Number of reflections | 3099 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 96.0 | 95.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | Ammonium sulphate, tris-HCl buffer, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
