1J9A
OLIGORIBONUCLEASE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 115 |
| Detector technology | CCD |
| Collection date | 2000-02-27 |
| Detector | BRUKER |
| Wavelength(s) | 0.9794, 0.9796, 0.9641, 0.9832 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 55.110, 55.110, 144.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.1851 |
| R-free | 0.29860 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.026 |
| Data reduction software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.040 | 0.150 |
| Number of reflections | 14507 | |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | Protein solution: 15 mg/mL protein in 0.01 M HEPES, 0.15 M sodium chloride, 0.005 M DTT, 0.005 M EDTA with pH 7.1-8.0. Reservoir: 2.3 M ammonium sulfate at pH 7.1-7.5. Hanging drop vapor diffusion method. Drops were formed by combining 4 microliters reservoir solution with 4 microliters protein solution. Room temperature., pH 7.3 |
