1J8Y

Signal Recognition Particle conserved GTPase domain from A. ambivalens T112A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	64.866, 128.020, 72.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.000
Rwork	0.227
R-free	0.25860
Structure solution method	MIR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.950
Rmerge	0.069 *
Total number of observations	450721 *
Number of reflections	21100
Completeness [%]	99.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	acetate	100 (mM)
3	1	reservoir	calcium acetate	100 (mM)
4	1	reservoir	PEG8000	18 (%)