1J8U
Catalytic Domain of Human Phenylalanine Hydroxylase Fe(II) in Complex with Tetrahydrobiopterin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.441, 108.676, 124.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.20300 |
| Starting model (for MR) | 1pah |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.030 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.051 | 0.370 |
| Total number of observations | 495560 * | |
| Number of reflections | 71940 | |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 | 277 | PEG2000, Na-hepes, ethylene glycol, pH 6.8, Vapor diffusion, hanging drop, anaerobically, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 10 (mM) | ||
| 2 | 1 | reservoir | dithiothreitol | 150 (mM) | |
| 3 | 1 | drop | protein | 5.5 (mg/ml) | |
| 4 | 1 | drop | sodium-Hepes | 20 (mM) | pH6.8 |
| 5 | 1 | drop | 75 (mM) |
