1J8T
Catalytic Domain of Human Phenylalanine Hydroxylase Fe(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM1A |
Synchrotron site | ESRF |
Beamline | BM1A |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.873 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.334, 108.419, 124.024 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.25200 |
Starting model (for MR) | 1pah |
RMSD bond length | 0.008 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 | 0.366 |
Total number of observations | 260644 * | |
Number of reflections | 48353 | 6776 * |
<I/σ(I)> | 6.4 | 2.1 |
Completeness [%] | 98.7 | 97.8 |
Redundancy | 5.4 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | PEG2000, Na-hepes, ethylene glycol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG2000 | 20 (%) | |
2 | 1 | reservoir | ethylene glycol | 10 (%(v/v)) | |
3 | 1 | reservoir | sodium HEPES | 0.12 (M) | pH6.8 |
4 | 1 | reservoir | dithiothreitol | 200 (mM) | |
5 | 1 | drop | protein | 5.5 (mg/ml) | |
6 | 1 | drop | sodium-Hepes | 20 (mM) | pH6.8 |
7 | 1 | drop | 75 (mM) |