1J8S

PAPG ADHESIN RECEPTOR BINDING DOMAIN-UNBOUND FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Detector technology	AREA DETECTOR
Spacegroup name	I 2 2 2
Unit cell lengths	55.130, 78.260, 158.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.570 - 2.100
R-factor	0.232
Rwork	0.232
R-free	0.25500
Structure solution method	MAD at the Se edge
RMSD bond length	0.006
RMSD bond angle	1.300
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.570	2.230
High resolution limit [Å]	2.100	2.100
Number of reflections	19087
Completeness [%]	93.3	95.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4-5 (mg/ml)
2	1	reservoir	PEG20000	4 (%(w/v))