1J8R

BINARY COMPLEX OF THE PAPG RECEPTOR-BINDING DOMAIN BOUND TO GBO4 RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Detector technology	AREA DETECTOR
Spacegroup name	I 2 2 2
Unit cell lengths	55.080, 79.672, 158.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.630 - 1.800
R-factor	0.22
Rwork	0.220
R-free	0.24200
Structure solution method	MAD at the Se edge
RMSD bond length	0.010
RMSD bond angle	1.600
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.630	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	29766
Completeness [%]	91.0	78.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4-5 (mg/ml)
2	1	reservoir	sodium citrate	100 (mM)
3	1	reservoir	ammonium acetate	0.5-1.1 (M)