1J8F

HUMAN SIRT2 HISTONE DEACETYLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	78.943, 119.070, 218.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.700
R-factor	0.235 *
Rwork	0.235
R-free	0.26000
Structure solution method	MIR
RMSD bond length	0.005 *
RMSD bond angle	1.480 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	1.700
Rmerge	0.041 *
Total number of observations	313019 *
Number of reflections	112374 *
Completeness [%]	98.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	used seeding *
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	used seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ethanol	24 (%(v/v))
2	1	reservoir		200 (mM)
3	1	reservoir	Bis-Tris-propane-HCl	100 (mM)