1J8B
Structure of YbaB from Haemophilus influenzae (HI0442), a protein of unknown function
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9789, 0.9790, 0.9500 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.390, 132.790, 36.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.750 |
| Rwork | 0.180 |
| R-free | 0.27400 * |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.046 * | 0.201 * |
| Total number of observations | 73832 * | |
| Number of reflections | 19416 * | |
| <I/σ(I)> | 18 | |
| Completeness [%] | 96.7 | 73.5 |
| Redundancy | 6.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | 1 M ammonium phosphate, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 3 | 1 | drop | dithiothreitol | 0.1 (mM) | |
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | reservoir | ammonium phosphate monobasic | 0.9-1.1 (M) | pH3.8 |
