1J89

HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), TETRAGONAL CRYSTAL FORM 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	110
Detector technology	CCD
Collection date	1995-01-01
Detector	CUSTOM-MADE
Wavelength(s)	0.920
Spacegroup name	P 43
Unit cell lengths	150.500, 150.500, 74.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.500 - 4.100
R-factor	0.273
Rwork	0.273
R-free	0.29100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1j88
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	4.250
High resolution limit [Å]	4.100	4.100
Rmerge	0.058	0.300
Total number of observations	25057 *
Number of reflections	11510
<I/σ(I)>	12.4
Completeness [%]	86.7	83.2
Redundancy	2.2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *	23 *	Garman, S.C., (2000) Nature, 406, 259. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris	100 (M)
3	1	reservoir	ammonium sulfate	1.4-1.6 (M)
4	1	reservoir	CHAPS	8 (mM)