1J89
HUMAN HIGH AFFINITY FC RECEPTOR FC(EPSILON)RI(ALPHA), TETRAGONAL CRYSTAL FORM 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1995-01-01 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.920 |
Spacegroup name | P 43 |
Unit cell lengths | 150.500, 150.500, 74.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.500 - 4.100 |
R-factor | 0.273 |
Rwork | 0.273 |
R-free | 0.29100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1j88 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 4.250 |
High resolution limit [Å] | 4.100 | 4.100 |
Rmerge | 0.058 | 0.300 |
Total number of observations | 25057 * | |
Number of reflections | 11510 | |
<I/σ(I)> | 12.4 | |
Completeness [%] | 86.7 | 83.2 |
Redundancy | 2.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 23 * | Garman, S.C., (2000) Nature, 406, 259. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | Tris | 100 (M) | |
3 | 1 | reservoir | ammonium sulfate | 1.4-1.6 (M) | |
4 | 1 | reservoir | CHAPS | 8 (mM) |