1J85

Structure of YibK from Haemophilus influenzae (HI0766), a truncated sequence homolog of tRNA (guanosine-2'-O-) methyltransferase (SpoU)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-01-27
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	40.370, 40.370, 165.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.195
R-free	0.26300
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.058	0.134
Total number of observations	113016 *
Number of reflections	9754
<I/σ(I)>	14
Completeness [%]	98.1	99.8
Redundancy	10.9	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	295	protein solution (12.5 mg/ml) in 50mM Tris-HCl pH7.5, 0.1mM EDTA, 0.1mM DTT; crystallization condition - 20 % polyethylene glycol monomethylether 2000, 0.1 M Na Acetate, 0.2 M Ammonium acetate, 3 % ethylene glycol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.5
3	1	drop	dithiothreitol	0.1 (mM)
4	1	drop	EDTA	0.1 (mM)
5	1	reservoir	PEG2000MME	20 (%)
6	1	reservoir	sodium acetate	0.1 (M)	pH4.6
7	1	reservoir	ammonium acetate	0.2 (M)
8	1	reservoir	ethylene glycol	3 (%)