1J83
STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798, 0.9796, 0.9593 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.264, 37.678, 71.234 |
| Unit cell angles | 90.00, 91.93, 90.00 |
Refinement procedure
| Resolution | 24.540 - 1.700 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.049 | 0.186 |
| Total number of observations | 249009 * | |
| Number of reflections | 37972 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 98.1 | 91.9 |
| Redundancy | 15.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 298 | AMMONIUM SULFATE, HEPES, PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.6 (mM) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 3 | 1 | reservoir | PEG400 | 2 (%(w/v)) |
