1J80

Osmolyte Stabilization of RNase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-08-26
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	44.600, 44.600, 98.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.204 *
Rwork	0.218
R-free	0.27000 *
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.300
High resolution limit [Å]	2.100	2.100
Rmerge	0.072	0.098
Redundancy	7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.75	293	Kim, E.E., (1992) Biochemistry, 31, 12304. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12.5 (mg/ml)
2	1	drop		6.0 (M)
3	1	drop	sodium acetate	0.1 (M)
4	1	reservoir	ammonium sulfate	80 (%sat)
5	1	reservoir	sodium acetate	0.1 (M)