1J7X
CRYSTAL STRUCTURE OF A FUNCTIONAL UNIT OF INTERPHOTORECEPTOR RETINOID-BINDING PROTEIN (IRBP)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9777,0.9790,0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.144, 65.756, 68.174 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.800 |
R-factor | 0.206 * |
Rwork | 0.205 |
R-free | 0.23000 * |
Structure solution method | MAD |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.234 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (V1.17) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.250 | 1.846 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.065 | 0.495 |
Number of reflections | 26579 | |
<I/σ(I)> | 8.4 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 4.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | PEG 8000, TRIS, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | PEG 8000, TRIS, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
2 | 1 | reservoir | 200 (mM) | ||
3 | 1 | reservoir | PEG8000 | 20 (%) |