1J7U
Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type IIIa AMPPNP Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 106 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.168, 91.321, 132.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.800 - 2.400 |
| R-factor | 0.217 * |
| Rwork | 0.217 |
| R-free | 0.27700 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.251 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.055 | 0.197 |
| Total number of observations | 24507 * | |
| Number of reflections | 92600 * | |
| <I/σ(I)> | 19.5 | 3 |
| Completeness [%] | 99.2 | 97.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, isopropanol, HEPES, magnesium chloride, AMPPNP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | magnesium sulfate | 1 (mM) | |
| 3 | 1 | drop | HEPES | 5 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | reservoir | HEPES | 100 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 7 | 1 | reservoir | 2-propanol | 10 (%(v/v)) |
