1J7N
Anthrax Toxin Lethal factor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.201, 137.461, 98.547 |
| Unit cell angles | 90.00, 98.35, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| Rwork | 0.229 |
| R-free | 0.26100 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.170 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.440 |
| High resolution limit [Å] | 2.200 | 2.300 |
| Number of reflections | 114503 | |
| <I/σ(I)> | 12.5 | 4.3 |
| Completeness [%] | 89.5 | 89.5 |
| Redundancy | 3.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | ammonium sulfate, TRIS, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | ammonium sulfate, TRIS, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
