1J7J
Crystal Structure of the HPRT from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-14 |
| Detector | MAR 165 mm CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.897, 83.897, 168.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 500.000 - 2.300 |
| Rwork | 0.210 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1TC1 without ligands and waters |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.792 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.301 |
| Number of reflections | 31306 | |
| <I/σ(I)> | 46.4 | 7.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.8 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 28% PEG 400, 200mM CaCl2, 100mM HEPES pH 7.5 |
