1J7G

Structure of YihZ from Haemophilus influenzae (HI0670), a D-Tyr-tRNA(Tyr) deacylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-01-01
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	55.470, 55.470, 128.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.640
Rwork	0.194
R-free	0.22000
Structure solution method	SAS
RMSD bond length	0.013
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.640	1.640
Rmerge	0.037	0.135
Total number of observations	352028 *
Number of reflections	25510
<I/σ(I)>	12.8
Completeness [%]	99.8	100
Redundancy	13	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	4 *	50 mM Tris, 0.1 mM DTT, 0.1 mM EDTA, pH 7.5, crystal formed during dialysis of protein (2.8mg/ml), temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	reservoir	Tris-HCl	50 (mM)	pH7.5
3	1	reservoir	dithiothreitol	0.1 (mM)
4	1	reservoir	EDTA	0.1 (mM)