1J7D

Crystal Structure of hMms2-hUbc13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-10
Detector	BRANDEIS - B1
Wavelength(s)	0.981958, 0.982127, 1.022130
Spacegroup name	P 21 21 21
Unit cell lengths	44.132, 74.137, 91.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.850
R-factor	0.215 *
Rwork	0.215
R-free	0.24430
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.540
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.024 *	0.263
Total number of observations	110669 *
Number of reflections	26066
<I/σ(I)>	37.1	3.2
Completeness [%]	98.3	89.5
Redundancy	4.25	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20-24 *	20% PEG 8000, 100mM Citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	HEPES	50 (mM)
3	1	drop		75 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop	dithiothreitol	0.5 (mM)
6	1	reservoir	PEG8000	20 (%(w/v))
7	1	reservoir	citrate	100 (mM)