1J78
Crystallographic analysis of the human vitamin D binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9116 |
| Spacegroup name | P 43 |
| Unit cell lengths | 132.248, 132.248, 73.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.380 - 2.310 |
| R-factor | 0.2201 * |
| Rwork | 0.220 |
| R-free | 0.25180 * |
| Structure solution method | COMBINED MAD AND MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.170 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.350 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.031 | 0.209 |
| Total number of observations | 187531 * | |
| Number of reflections | 55444 | |
| <I/σ(I)> | 22.7 | 5.2 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 293 | Verboven, C.C., (1995) J. Steroid Biochem. Mol. Biol., 54, 11. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 7.5 (%(v/v)) | |
| 2 | 1 | reservoir | acetate | 0.1 (M) | pH4.6 |
| 3 | 1 | drop | protein | 30 (mg/ml) |
