1J75
Crystal Structure of the DNA-Binding Domain Zalpha of DLM-1 Bound to Z-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9102 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 63.611, 63.611, 72.238 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.000 - 1.850 |
| R-factor | 0.22152 * |
| Rwork | 0.218 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qbj |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.590 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.066 | 0.613 |
| Total number of observations | 182894 * | |
| Number of reflections | 7802 | |
| <I/σ(I)> | 6.7 | 2.9 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 23.4 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 24 * | 15% PEG 4000, 0.1M ammonium hydrogen phosphate, 15% ethylene glycol, 0.1M MES pH 6.0, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | ammonium hydrogen phosphate | ||
| 3 | 1 | 1 | ethylene glycol | ||
| 4 | 1 | 1 | MES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 5 (mM) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | protein | 2 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
| 5 | 1 | reservoir | MES | 100 (mM) | |
| 6 | 1 | reservoir | ammonium phosphate | 100 (mM) | |
| 7 | 1 | reservoir | ethylene glycol | 15 (%(v/v)) |
