1J6X
CRYSTAL STRUCTURE OF HELICOBACTER PYLORI LUXS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9795, 0.9641 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.140, 71.140, 130.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.380 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.490 |
| High resolution limit [Å] | 2.380 | 2.400 |
| Rmerge | 0.056 * | 0.198 * |
| Number of reflections | 13672 | |
| <I/σ(I)> | 29.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 20 * | PEG 1000, Ammonium sulfate, MES, BME, NaCl, pH 5.75, VAPOR DIFFUSION, HANGING DROP at 293K, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG1000 | 32 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 3 | 1 | reservoir | MES | 100 (mM) | |
| 4 | 1 | reservoir | beta-mercaptoethanol | 30 (mM) | |
| 5 | 1 | drop | protein | 5 (mg/ml) |
