1J5U
CRYSTAL STRUCTURE OF AN ARCHEASE, POSSIBLE CHAPERONE (TM1083) FROM THERMOTOGA MARITIMA AT 2.0 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.925223, 0.978645, 0.979029 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 105.280, 105.280, 105.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.090 - 2.000 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.27100 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.540 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.176 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 13851 | |
| <I/σ(I)> | 16.8 | 4.9 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 5.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 0.14 M CaCl2; 14 % isoprop; 0.07 M Acetate pH 4.6, 30 % Glycerol, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K, pH 4.60 |
