1J5T
Crystal structure of indole-3-glycerol phosphate synthase (TM0140) from Thermotoga maritima at 3.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-20 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 117.597, 117.597, 82.153 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.290 - 3.000 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.480 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.293 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 6026 | |
| <I/σ(I)> | 21 | 6.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 293 | 50% PEG 200, 0.1M phosphate-Citrate ph 4.2, 0.2M NaCl, pH 5.2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K, pH 5.20 |
