1J5P
Crystal structure of aspartate dehydrogenase (TM1643) from Thermotoga maritima at 1.9 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.93218, 0.979181, 0.978932 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.200, 63.200, 126.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.690 - 1.900 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.717 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 23895 | |
| <I/σ(I)> | 15.6 | 1.4 |
| Completeness [%] | 98.5 | 88.6 |
| Redundancy | 6.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 20% PEG 8000, 0.05M KH2PO4, temperature 293K |
