1J59
CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
Replaces: 1BERExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-11-01 |
| Detector | FUJI |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 136.990, 152.800, 76.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.27900 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 22.900 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.115 |
| Total number of observations | 165000 * |
| Number of reflections | 23876 |
| <I/σ(I)> | 2 |
| Completeness [%] | 84.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 19 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES-NaOH | 50 (mM) | pH6.5 |
| 10 | 1 | reservoir | PEG3350 | 6.5 (%(w/v)) | |
| 11 | 1 | drop | CAP | 0.1-0.2 (mM) | |
| 12 | 1 | drop | DNA | 1.2-1.5fold molar excess | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | 0.02 (%(w/v)) | ||
| 6 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 7 | 1 | reservoir | spermine | 2 (mM) | |
| 8 | 1 | reservoir | cAMP | 2 (mM) | |
| 9 | 1 | reservoir | n-octyl-beta-D-glucopyranoside | 0.3 (%(w/v)) |
