1J4E
FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE COVALENTLY BOUND TO THE SUBSTRATE DIHYDROXYACETONE PHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2001-03-01 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.229, 99.388, 84.484 |
| Unit cell angles | 90.00, 98.29, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.650 |
| R-factor | 0.213 * |
| Rwork | 0.213 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ald |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.303 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.034 * | 0.220 * |
| Number of reflections | 35634 | |
| <I/σ(I)> | 20 | 10 |
| Completeness [%] | 86.0 | 60 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 18 * | pH 7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | pH7.4 |
| 3 | 1 | drop | dithiothreitol | 0.1 (mM) | |
| 4 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | PEG | 10 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.4 |
| 8 | 1 | reservoir | PEG6000 | 28 (%) |
