1J40
Direct observation of photolysis-induced tertiary structural changes in human haemoglobin; Crystal structure of alpha(Ni)-beta(Fe-CO) hemoglobin (laser unphotolysed)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.708, 94.860, 99.730 |
Unit cell angles | 90.00, 101.97, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.450 |
R-factor | 0.1664 |
Rwork | 0.165 |
R-free | 0.21100 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.310 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.22) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.067 * | 0.213 * |
Total number of observations | 751479 * | |
Number of reflections | 200565 | |
Completeness [%] | 95.9 | 77.3 * |
Redundancy | 3.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.6 | 293 | PEG 4000, 50mM citrate-ammonium buffer, pH 6.6, SMALL TUBES, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | CO-saturated | 1-2 (%(w/v)) | |
2 | 1 | 1 | PEG3350 | 18 (%(w/v)) | |
3 | 1 | 1 | 0.15 (M) | ||
4 | 1 | 1 | homocysteine | 5 (mM) | |
5 | 1 | 1 | citrate-ammonium | 50 (mM) | pH6.6 |