1J3H
Crystal structure of apoenzyme cAMP-dependent protein kinase catalytic subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 97 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.776, 143.601, 62.809 |
| Unit cell angles | 90.00, 105.71, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.900 |
| Rwork | 0.257 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctp |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.900 |
| High resolution limit [Å] | 2.900 * | 2.800 |
| Rmerge | 0.055 | 0.493 * |
| Total number of observations | 102700 * | |
| Number of reflections | 18800 * | |
| Completeness [%] | 91.8 | 82.8 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | Tris-hydrogen chloride, MPD, Bicine, ammonium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 0.1 (mM) | pH7.5 |
| 2 | 1 | reservoir | MPD | 10-20 (%(v/v)) | |
| 3 | 1 | drop | MPD | 3 (%(v/v)) | |
| 4 | 1 | drop | Bicine | 0.1 (mM) | pH8.0 |
| 5 | 1 | drop | protein | 7.5 (mg/ml) |
