1J39

Crystal Structure of T4 phage BGT in complex with its UDP-glucose substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	150.391, 51.431, 52.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.870
Rwork	0.194
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bgt
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.940
High resolution limit [Å]	1.870	1.870
Rmerge	0.047 *	0.239 *
Number of reflections	34078 *
<I/σ(I)>	11.3	3.3
Completeness [%]	98.6	96.3
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292	ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	0.8 (M)
2	1	drop	Tris-HCl	50 (mM)	pH8.5
3	1	reservoir	ammonium sulfate	1.6 (M)