1J38

Crystal Structure of Drosophila AnCE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	123
Detector technology	CCD
Collection date	2000-12-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9280
Spacegroup name	P 1 21 1
Unit cell lengths	94.912, 121.223, 94.740
Unit cell angles	90.00, 99.39, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.246
Rwork	0.246
R-free	0.28100
Structure solution method	MIR
RMSD bond length	0.008 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.063 *	0.234 *
Total number of observations	442229 *
Number of reflections	66143
Completeness [%]	92.8	81.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	22 *	Sodium Formate 7.2M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-25 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	drop		0.2 (M)	pH7.5
4	1	reservoir	sodium formate	7.2 (M)
5	1	reservoir		2 (mM)