1J37
Crystal Structure of Drosophila AnCE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2000-12-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9280 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.912, 121.223, 94.740 |
| Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.28300 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.059 * | 0.375 * |
| Total number of observations | 590560 * | |
| Number of reflections | 82133 | |
| Completeness [%] | 90.8 | 76.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 22 * | Sodium Formate 7.2M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-25 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 0.2 (M) | pH7.5 | |
| 4 | 1 | reservoir | sodium formate | 7.2 (M) | |
| 5 | 1 | reservoir | 2 (mM) |
