1J32
Aspartate Aminotransferase from Phormidium lapideum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.835 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 120.220, 131.260, 107.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.070 - 2.100 |
| Rwork | 0.198 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.070 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.077 | 0.255 |
| Number of reflections | 49991 | |
| <I/σ(I)> | 7.3 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 296 | cacodylate, calcium acetate, PEG 8000 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
