1J2X

Crystal structure of RAP74 C-terminal domain complexed with FCP1 C-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-18
Detector	MARRESEARCH
Wavelength(s)	0.97899
Spacegroup name	C 1 2 1
Unit cell lengths	59.605, 30.446, 47.379
Unit cell angles	90.00, 106.38, 90.00

Refinement procedure

Resolution	22.700 * - 2.000
R-factor	0.207
Rwork	0.203
R-free	0.25500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i27
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.045	0.164
Total number of observations	29692 *
Number of reflections	5657
<I/σ(I)>	18.1	5.3
Completeness [%]	99.8	97.8
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEGMME 550, zinc sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH6.5
3	1	reservoir	zinc sulfate heptahydrate	10 (mM)
4	1	reservoir	PEG550	25 (%(v/v))