1J2X
Crystal structure of RAP74 C-terminal domain complexed with FCP1 C-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97899 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.605, 30.446, 47.379 |
| Unit cell angles | 90.00, 106.38, 90.00 |
Refinement procedure
| Resolution | 22.700 * - 2.000 |
| R-factor | 0.207 |
| Rwork | 0.203 |
| R-free | 0.25500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i27 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.045 | 0.164 |
| Total number of observations | 29692 * | |
| Number of reflections | 5657 | |
| <I/σ(I)> | 18.1 | 5.3 |
| Completeness [%] | 99.8 | 97.8 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEGMME 550, zinc sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
| 3 | 1 | reservoir | zinc sulfate heptahydrate | 10 (mM) | |
| 4 | 1 | reservoir | PEG550 | 25 (%(v/v)) |
