1J2W

Tetrameric Structure of aldolase from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-11-25
Detector	RIGAKU RAXIS V
Wavelength(s)	0.8
Spacegroup name	P 21 21 21
Unit cell lengths	64.280, 97.686, 137.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.500
R-factor	0.198
Rwork	0.197
R-free	0.21300
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.290 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.067 *	0.456 *
Total number of observations	665297 *
Number of reflections	137192 *
Completeness [%]	98.5 *	99.5
Redundancy	4.85 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	4.9 *	295	MPD, Tris HCl, magnesium chloride, pH 7.9, MICROBATCH, temperature 295.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	ammonium sulfate	1 (M)
3	1	1	sodium acetate	1 (M)
4	1	1	MPD	30 (%)	pH4.9