1J2W
Tetrameric Structure of aldolase from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-11-25 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.8 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.280, 97.686, 137.918 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.500 |
R-factor | 0.198 |
Rwork | 0.197 |
R-free | 0.21300 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.290 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.067 * | 0.456 * |
Total number of observations | 665297 * | |
Number of reflections | 137192 * | |
Completeness [%] | 98.5 * | 99.5 |
Redundancy | 4.85 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 4.9 * | 295 | MPD, Tris HCl, magnesium chloride, pH 7.9, MICROBATCH, temperature 295.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 20 (mg/ml) | |
2 | 1 | 1 | ammonium sulfate | 1 (M) | |
3 | 1 | 1 | sodium acetate | 1 (M) | |
4 | 1 | 1 | MPD | 30 (%) | pH4.9 |