1J2V

Crystal Structure of CutA1 from Pyrococcus Horikoshii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-11
Detector	MARRESEARCH
Wavelength(s)	0.9000, 0.9792, 0.9794
Spacegroup name	P 63
Unit cell lengths	52.569, 52.569, 58.132
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.000
Rwork	0.239
R-free	0.28700
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	6154
<I/σ(I)>	8.4	6.9
Completeness [%]	98.9	99.3
Redundancy	11.2	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	Ammonium Sulfate , Sodium Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K