1J2T
Creatininase Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.989, 150.742, 167.066 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.17592 |
| Rwork | 0.175 |
| R-free | 0.19100 * |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.037 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.331 |
| Number of reflections | 237380 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 5.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.5M Li sulfate, 0.1M Na HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |
| 3 | 1 | reservoir | lithium sulfate | 1.5 (M) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
