1J2Q

20S proteasome in complex with calpain-Inhibitor I from archaeoglobus fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-05
Detector	MARRESEARCH
Wavelength(s)	1.1
Spacegroup name	P 31 2 1
Unit cell lengths	148.097, 148.097, 303.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.990 - 2.800 *
R-factor	0.243
Rwork	0.241
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.880
High resolution limit [Å]	2.800	2.830
Rmerge	0.071 *	0.399 *
Total number of observations	1140521 *
Number of reflections	87322
Completeness [%]	94.9	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	24 *	9% PEG 400, 80mM MgCl2, 100mM NaAc, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir		100 (mM)	pH4.6
3	1	reservoir	PEG400	9 (%(w/v))
4	1	reservoir		80 (mM)