1J2Q
20S proteasome in complex with calpain-Inhibitor I from archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-05 |
Detector | MARRESEARCH |
Wavelength(s) | 1.1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 148.097, 148.097, 303.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.990 - 2.800 * |
R-factor | 0.243 |
Rwork | 0.241 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.880 |
High resolution limit [Å] | 2.800 | 2.830 |
Rmerge | 0.071 * | 0.399 * |
Total number of observations | 1140521 * | |
Number of reflections | 87322 | |
Completeness [%] | 94.9 | 95.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 24 * | 9% PEG 400, 80mM MgCl2, 100mM NaAc, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | reservoir | 100 (mM) | pH4.6 | |
3 | 1 | reservoir | PEG400 | 9 (%(w/v)) | |
4 | 1 | reservoir | 80 (mM) |