1J2E
Crystal structure of Human Dipeptidyl peptidase IV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.040, 125.920, 136.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| Rwork | 0.249 |
| R-free | 0.30150 * |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.305 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.078 | 0.213 |
| Total number of observations | 385015 * | |
| Number of reflections | 59988 * | |
| <I/σ(I)> | 7.5 | 3.5 |
| Completeness [%] | 95.0 * | 72.8 |
| Redundancy | 6.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 * | 293 | Hiramatsu, H., (2003) Acta Cryst., D59, 595. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Gly-NaOH | 71 (mM) | pH8.7 |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | reservoir | Gly-NaOH | 180 (mM) | pH9.5 |
| 4 | 1 | reservoir | sodium acetate | 180 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 18 (%) |
