1J2E

Crystal structure of Human Dipeptidyl peptidase IV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	118.040, 125.920, 136.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.600
Rwork	0.249
R-free	0.30150 *
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.305 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.078	0.213
Total number of observations	385015 *
Number of reflections	59988 *
<I/σ(I)>	7.5	3.5
Completeness [%]	95.0 *	72.8
Redundancy	6.4	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.7 *	293	Hiramatsu, H., (2003) Acta Cryst., D59, 595. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Gly-NaOH	71 (mM)	pH8.7
2	1	drop	protein	20 (mg/ml)
3	1	reservoir	Gly-NaOH	180 (mM)	pH9.5
4	1	reservoir	sodium acetate	180 (mM)
5	1	reservoir	PEG4000	18 (%)