1J2C
Crystal structure of rat heme oxygenase-1 in complex with biliverdin IXalpha-iron cluster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.400, 65.400, 121.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 2.400 |
| R-factor | 0.199 |
| Rwork | 0.194 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ix3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.140 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 * | 0.343 * |
| Total number of observations | 88285 * | |
| Number of reflections | 12207 | |
| <I/σ(I)> | 7.1 | 2.2 |
| Completeness [%] | 99.7 | 99.7 * |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sugishima, M., (2002) J.Biol.Chem., 277, 45086. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 4 (M) | |
| 2 | 1 | drop | protein | 18 (mg/ml) | |
| 3 | 1 | drop | potassium phosphate | 50 (mM) | pH7.0 |
| 4 | 1 | drop | sodium azide | 5 (mM) |
