1J2B

Crystal Structure Of Archaeosine tRNA-Guanine Transglycosylase Complexed With lambda-form tRNA(Val)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	30
Detector technology	CCD
Collection date	2002-06-13
Detector	MARRESEARCH
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	230.834, 230.834, 269.255
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 3.300
R-factor	0.225
Rwork	0.225
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iq8
RMSD bond length	0.012
RMSD bond angle	1.570 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.099	0.487
Total number of observations	334265 *
Number of reflections	41049 *
<I/σ(I)>	16.7	2.31
Completeness [%]	99.7	99.8
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	20 *	citrate, ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	citrate
2	1	1	ammonium phosphate
3	1	2	citrate
4	1	2	ammonium phosphate

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-8 (mg/ml)
2	1	drop	citrate	100 (mM)	pH5.6
3	1	drop	ammmonium dihydrogen phosphate	1.0 (M)
4	1	drop	xylitol	0.3 (%)
5	1	reservoir	citrate	100 (mM)	pH5.6
6	1	reservoir	ammmonium dihydrogen phosphate	1.0 (M)
7	1	reservoir		400 (mM)