1J2A
Structure of E. coli cyclophilin B K163T mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2000-10-06 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.550, 82.550, 52.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| Rwork | 0.201 |
| R-free | 0.22500 |
| Structure solution method | MIR |
| Starting model (for MR) | 1J28 |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 0.842 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 0.402 |
| Total number of observations | 133992 * | |
| Number of reflections | 19219 | |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 99.0 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 38 (%(w/v)sat) | |
| 3 | 1 | drop | 0.04 (%(w/v)) | ||
| 4 | 1 | drop | Tris-HCl | 80 (mM) | pH8.0 |
| 5 | 1 | reservoir | ammonium sulfate | 46 (%(w/v)sat) | |
| 6 | 1 | reservoir | 0.04 (%(w/v)) | ||
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
