1J20
Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with product
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 228.780, 228.780, 159.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 2.000 |
Rwork | 0.212 |
R-free | 0.24400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.610 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.054 * | 0.254 * |
Total number of observations | 670809 * | |
Number of reflections | 209661 | |
Completeness [%] | 99.4 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.5 | 293 | tris, ammonium sulfate, glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
3 | 1 | reservoir | glycerol | 30 (%(v/v)) | |
4 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |