1J1T
Alginate lyase from Alteromonas sp.272
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS V |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 46.200, 46.200, 387.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.800 - 2.000 |
| Rwork | 0.194 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.800 |
| High resolution limit [Å] | 1.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 5.5 | 277 | PEG 8000, Ammomium Sulfate, pH 5.5, LIQUID DIFFUSION, temperature 277K |
| 1 | LIQUID DIFFUSION | 5.5 | 277 | PEG 8000, Ammomium Sulfate, pH 5.5, LIQUID DIFFUSION, temperature 277K |
