1J1G
Crystal structure of the RNase MC1 mutant N71S in complex with 5'-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-01 |
| Detector | OXFORD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.542, 66.209, 75.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.810 - 1.600 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bk7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.810 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.157 | 0.233 |
| Number of reflections | 25113 | |
| <I/σ(I)> | 8.6 | 1.4 |
| Completeness [%] | 95.6 | 72.9 |
| Redundancy | 5.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M sodium acetate, 0.1M sodium cacodylate, 24% PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
